部分中国砂梨和日本梨的RAPD分析

RAPD Analysis for Genetic Assessment of Some Cultivars of Pyrus pyrifolia Derived from China and Japan

采用RAPD分子标记技术,对来自福建和浙江的6份中国砂梨和36份日本梨的亲缘关系进行了分析。27个引物产生了可记录的多态性条带261条。聚类分析将这些梨品种和类型分为4个中国砂梨群和5个日本梨群。其中4个日本梨群中都包含有日本高知县的品种或类型。尽管由于取样的偏差,中国砂梨和日本梨在聚类分析中没有混合聚为大群,但中国砂梨品种与部分日本梨,特别是来自高知县的日本梨的相似系数高于与中国梨之间的相似系数。

The present study, using RAPD analysis, was undertaken to characterize genetic variation in Japanese pears and Chinese sand pears, as well as their genetic relationships. The materials used consisted of 36 Japanese pears and 6 Chinese accessions from Zhejiang and Fujian provinces. A total of 27 primers generated 261 scorable polymorphic RAPD bands. Clustering analysis revealed 9 groups： 4 consisting of Chinese sand pears and 5 from Japan, of which 4 Japanese pear groups contained cultivars or types from Kouchi Prefecture, Japan. Chinese sand pears and Japanese pears were not mingled together into groups due to sampling bias. However, Chinese sand pear cultivars showed higher affinities to some Japanese pears, especially those from Kouchi prefecture than to Chinese ones.