一种消除维生素C干扰的血清尿酸检测方法

A method for eliminating the interference of vitamin C in the determination of serum uric acid

目的 利用4-羟基-2，2，6，6-四甲基-1-氧-哌啶（AAQ-2）作为维生素C（Vit C）的熄灭剂，建立双试剂终点法测定血清尿酸（UA），以消除VitC在UA测定中的干扰。方法 采用双试剂终点法测定UA。结果 方法重复性：日内相对标准差（RSD）为1．50％，1．37％，日间RSD为1．55％、1．40％；平均回收率：99．34％；本法线性可达50～1550μmol／L。结论 本方法可消除血清中高浓度VitC对UA测定的干扰，且适用于自动分析仪。

Objective Using 4-hydroxy-2,2,6,6-tetramethyl-1-piperidinyloxy free radical（ ascorbic acid quencher-2,AAQ-2） as quencher of vitamin C（ Vit C）, to establish an end point method of double reagent for determining serum uric acid（ UA）, to eliminate the interference of Vit C in determining serum UA. Methods The level of serum UA was determined by end point method of double reagent. Results The assay precision was satisfactory. Within-run relativestandard deviation（RSD） were 1.50% and 1.37%, between-run RSD were 1.55% and 1.40% respectively. The average rate of recovery was 99.34%. The assay linearity was 50-1 550 μmol/L. Conclusions The method can eliminated the interference of high concentration Vit C in determining serum UA,and be used in automatic analysis.