FK506对肝移植受者外周血T细胞亚群及共刺激分子表达调节的研究

Study on the regulatory effect of FK506 on T cell subgroups and the co-stimulator expression of allo-liver recipients

目的通过观察他克莫司(FK506)对肝移植患者T细胞亚群及T细胞表面CD28、CD152和可诱导共刺激分子(ICOS)表达的影响,探讨FK506对细胞信号传导通路的作用。方法流式细胞技术测定健康对照组、终末期肝脏疾病患者组及使用FK506治疗的肝移植患者组T细胞亚群及各亚群细胞表面CD28、CD152和ICOS的表达。结果CD3+T细胞总数在3组间差异无显著性;与疾病对照组比较,治疗组CD4+T细胞数明显下降,CD8+T细胞数明显增加;CD4+T细胞和CD8+T细胞上CD28和ICOS的表达均显著降低,而CD152分子则均显著升高。结论FK506对治疗组患者T细胞亚群平衡紊乱的迅速纠正有重要作用。通过抑制正性共刺激分子并同时促进负性共刺激分子表达,FK506可在T细胞免疫信号传导通路的上游阻断T细胞活化信号的传导,发挥了免疫调节功能。

Objective Through observing the influence of FKS06 on T cell subgroups and the expression of costimulators CD28,CD152 and inducible costimulator（ICOS） in patients receiving allo-liver transplantation and FKS06 treatment, to explore the effect of FKS06 on T cell signal transduction. Methods The T cell subgroups and the expression of CD28, CD152 and ICOS on peripheral T cells in healthy-control group, disease-control group and treatment group were determined by flowcytometry. Results The total number of T lymphocytes were not significantly different among three groups. Compared with disease-control group, the number of CD4^＋ T cell of the treatment group was much lower and the number of CD8^＋T cell was much higher; The expressions of CD28 and ICOS significantly decreased whilethe CD152 expression obviously increased. Conclusions FK506 plays an important role in the quick correction of the turbulence of T cell subgroup balance. Through inhibiting the expression of positive costimulators and promoting the expression of negative eostimulator CD152, FK506 blocks the activating signal transduction at the upper stream of T cell immune signal pathway and thus inhibits the activation of T cell.