儿科患儿使用CD-1700血液分析仪检测中间型细胞镜检的探讨

Study on the rule of microscopic examination for mononuclear cell in blood of pediatric patients detected by CD-1700 hematology analyzer

目的 探讨三分群CD-1700血液分析仪在儿科患儿血常规检查时中间型细胞（MO）镜检复查规则，以保证检验结果的正确性。方法 收集232例临床血常规标本进行全自动血液分析仪检测，同时进行外周血涂片人工镜检，将仪器白细胞分类的MO结果与人工镜检分类结果进行对比统计，分析差异和原因。结果 将仪器分类的MO结果分为3组，与人工镜检分类结果比较，MO在〈8％时，r=0．804，P〉0．05；MO在8％～9．9％时，r=0．796，P〉0．05。以上2组标本2种方法间差异无显著性，有良好的相关性。MO≥10％时，r=0．352，P〈0．05，2种方法间差异显著，相关性差，同时该组标本的人工镜检异常结果检出率（15．5％）明显高于前2组（2．1％和1．9％）。结论 当血液分析仪检测白细胞分类MO为10％以上时，均应作瑞特染色人工镜检复查，以防止异型淋巴细胞、嗜酸性粒细胞、幼稚细胞等病理性细胞漏检。

Objective To study the rule of microscopic examination for mononuclear（MO） cell in blood of pediatric patients detected by the CD-1700 hematology analyzer and assure the quality of blood routine examination. Methods MO cell in 232 pediatric patients was determined by the hematology analyzer and microscopic examination of the bloodsmear. Results If MO cell level was 〈 10% , there was no significant difference between the microscopic examination and instrument analysis. If MO cell level was i〉 10% ,there was significant difference between two methods, and 15.5% sample with abnormal cell was detected by microscopic method. Conclusions It should make the microscopic examination when the level of MO cell is over 10% by hematology analyzer,in order to detect the abnormal cell such as atypical lymphocyte, eosinophil cell,immature cell etc.