人乳头瘤病毒感染对宫颈腺癌中p27^kip1蛋白表达的影响

Effect of HPV infection in cervical adenocarcinoma on the expressions of p27^kip1 protein

目的探讨人乳头瘤病毒（HPV）感染对宫颈腺癌中细胞周期调控因子p27^kip1蛋白表达的影响以及二者在宫颈腺癌发生过程的作用。方法采用原位杂交和免疫组化（二步法）检测24例慢性宫颈炎和86例宫颈腺癌组织的HPV16／18DNA和p27^kip1蛋白表达情况。结果HPV16/18DNA在宫颈腺癌组织中的阳性率为65．1％，显著高于慢性宫颈炎组织8．3％（P〈0．01）。p27^kip1蛋白表达在宫颈腺癌组织中的阳性率为55．8％，明显低于慢性宫颈炎组织79.2％（P〈0．05）．HPV16／18感染与宫颈腺癌的组织学类型无关，但与p27^kip1表达呈负相关（P〈0．05）。p27^kip1表达与官颈腺癌组织学类型有明显相关性，透明细胞腺癌p27^kip1阳性表达率明显低于其它类型宫颈腺癌（P〈0．05）。结论HPV16／18感染可能引起p27^kip1蛋白表达异常，从而导致宫颈腺癌发生。

Objective To study the effect of human papilloma virus （HPV） infection in cervical adenocarcinoma on the expressions of p27^kip1 protein, and to analyse the role of HPV infection and p27^kip1 protein expression to the carcinogenesis of cervical adenocarcinoma. Methods The expressions of HPV16/18DNA and p27^kip1 protein were determined by in situ hybridization and immunohistochemistry in 86 cases of cervical adenocarcinoma and 24 cases of cervical chronic inflammation. Results The positive rates of HPV16/18DNA in cervical adenocarcinoma were 65.1%, which significantly higher than that in cervical chronic inflammation 8.3% （P〈0.01）. The positive rate of p27^kip1 protein in cervical adenocarcinoma was 55.8%, which significantly lower than that in cervical chronic inflammation 79.2% （P〈0.05）. There was no relationship between HPV16/18 infection and the histological subtypes. There was negative correlation between HPV 16/18 infection and p27^kip1 expression. The expressions of p27^kip1 were related to the histological types, the level of p27^kip1 was significantly lower in clear-cell adenocarcinoma than in other types （P〈0.05）. Conclusions HPV16/18 infection may produce the abnormal expression of p27^kip1 protein and lead to the genesis of cervical adenocarcinoma.