透明质酸钠对兔心脏术后心包粘连炎性因子影响

Effect of Sodium Hyaluronate on Inflammatory Cytokine in Rabbit's Postoperative Pericardial Adhesion

目的观察透明质酸钠在兔心脏术后心包粘连对血清中肿瘤坏死因子α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)含量的影响。方法采用兔心脏术后心包粘连模型。用药各组分别给予:①术后心包腔灌注透明质酸钠(5mg/只),每日1次,共7天;②;模型组及假手术组给予等容积的生理盐水。各组动物于术后6h、24h、3d、5d共4个时间点静脉取血,测定IL-1β、IL-6、TNF-α炎性细胞因子的含量。结果SHA组IL-1β、IL-6、TNF-α含量与Model组相比均在高峰期明显降低(P<0.01)。结论透明质酸钠对兔心脏术后心包粘连的形成有干预作用;其干预作用可能与降低血清中IL-1β、IL-6、TNF-α的含量有关。

Objective To evaluate the effects of Sodium Hyaluronate （SHA） on inflammatory cytokine .TNF-α ,IL - β, IL -6 in rabbit＇s postoperative pericardial adhesion. Methods Rabbits were randomly divided into three groups, we used the rabbit model with pericardial adhesions, ①5mg SHA were injected to pericardial cave of each rabbit after the operation, qd , 7 days ; ②equal quantity of nαmal saline were injected in model group and sham operation group. IL - 1β, IL - 6 and TNF - α were tested at 6h,24h,3d,5d time point. Results Group SHA decreased mαe significantly the IL - 1β, IL- 6 and TNF-α maximum levels than group Model and group Sham （ P 〈0.01 ）. Conclusion SHA could effectively prevent postoperative pericardial adhesion and reduce the serum levels of IL - 1β, IL - 6 and TNF - α. The protective effect is, to some degree, related to the inhibition of IL - 1β, IL - 6 and TNF - α.