摘要
莲子草假隔链格孢在改良Fries培养基中能够产生使空心莲子草致病的毒素，培养滤液经DM-130大孔树脂吸附-甲醇洗脱-蒸干-乙酸乙酯浸提，获得毒素粗提物。经HPLC分析，毒素粗提物由5个组分组成，其中最大的吸收峰是保留时间为9．520min的组分。基本性质研究结果表明，毒素为非蛋白类、相对分子质量在10000以下的小分子物质，在121℃和pH1～11的条件下能够稳定存在。
Nimbya alternantherae could produce phytotoxin in modified Fries culture liquid. The filtrate was chromatographed on a DM-130 resin column eluting with methanol, and evaporation of the solvent gave a brown mush. The active fractions were then extracted with ethyl acetate. 5 fractions were obtained through high performance liquid chromatography（HPLC）. The fraction with a retention time of 9.520 min was the most abundant one. It was a non- protein metabolic product with a relative molecular mass less than 10,000, and kept steady at the temperature of 121℃ and in pH between 1 to 11.
出处
《化学世界》
CAS
CSCD
北大核心
2006年第7期414-416,394,397,共5页
Chemical World
基金
国家"十五"攻关项目(2001BA509B07)
广东省科技计划项目(2002C20508)
关键词
莲子草假隔链格孢
空心莲子草
毒素
组成
性质
Nimbya alternantherae
Alternanthera philoxeroides
phytotoxin
composition
properties
