制炭温度及受热时间对槐花高效液相指纹图谱的影响

The effects of carbonizing temperature and heating time on HPLC fingerprints of Flos Sophorae

目的:考察制炭温度及受热时间对中药槐花高效液相指纹图谱的影响,为槐花炭炮制工艺的规范化研究提供实验数据。方法:采用恒温烘箱为加热设备,从120°C至250°C间隔10°C共14个温度,每个温度下对槐花分别加热30 m in或60 m in,共得到28份样品;以高效液相色谱法分别测定每份样品的指纹图谱。选择生品的指纹图谱为对照图谱,分析各样品指纹图谱的变化。结果:受热30 m in的样品,在120°C^150°C受热时,指纹图谱均未发生变化;在170°C受热时,其指纹图谱均略有变化;180°C^190°C受热时,其指纹图谱均发生显著性变化。200°C以上受热,其指纹图谱均显示化学成分被破坏。160°C以上受热60 m in的样品对温度的敏感性高于受热30 m in的样品。结论:综合考虑指纹图谱变化及能源合理利用,槐花制炭温度以185°C±2°C、加热30 m in为宜。

AIM： To explore the effects of carbonizing temperature and time on HPLC fingerprints of Flos Sophorae for improving the processing technics of Flos Sophorae carbonized. METHODS： Samples were prepared by an oven for different time and at different temperatures, separately. Then the fingerprints of the samples were determined by HPLC, separately. According to the fingerprints of the crude drug, the differences between the fingerprints of the samples were compared. RESULTS ： Heated for 30 min, there was no significant change of the fingerprints of the samples which were heated at 120 ℃-150 ℃; there was a little change in the fingerprints when the samples were heated at 170 ℃, but the significant change was observed when they were heated at 180 ℃-190 ℃. The fingerprints showed the chemical constitutents were almost destroyed over 200 ℃. Over 160 ℃, the effects on the samples which were heated for 60 min were more obviously than on those which were heated for 30 min. CONCLUSION： According to the change of the fingerprints and in view of the efficient usage of the energy, Flos Sophorae carbonizing should be heated at 185 ℃ ±2 ℃ and for 30 min.