红鳍东方鲀三倍体诱导的初步研究

Triploidy Induction of Takifugu rubripes by Cold Shock

通过冷休克的方法诱导红鳍东方鲀的受精卵,孵化后的仔鱼,用PAS-Ⅲ型细胞流速仪测定其倍性,得到较高的三倍体诱导率。受精后8 min,0℃下处理15 min和受精后5 min,2℃下处理15min三倍体率最高,为100%;受精后8 min,4℃下处理10 min倍化率最低,为20%;其余几组的三倍体率为90%。正交试验结果表明,影响三倍体诱导率的主次顺序是处理时间、处理温度、处理起始时间。

The triploidy of Takifugu rupripes was induced by cold shock in this experiment. After the fertilized eggs were induced, they are incubated in sea water at a water temperature of 19 ℃ and hatched. The triploid rate was measured in DNA content in nuclei by flow cytometry. The maximum triploidy rate （ 100% ） was observed 8 minutes after fertilization, for 15 minute treatment at 0 ℃, and 5 minutes after fertilization, for 15 minute treatment at 2 ℃ ; while the minimum triploidy rate （20%） occurred 8 minutes after fertilization, for 10 minute treatment at 4 ℃. A triploidy rate of 90% was found in other treatments. The orthogonal design results showed that the triploidy rate was primarily affected by treatment duration, followed by treatment temperature and treatment duration