摘要
目的建立用高效液相色谱(HPLC)法测定绿梅止泻颗粒中芦丁含量的方法。方法采用Dalian Elite Hypersil ODS2(200inlnx4.6mm,5μm)柱,以甲醇.水.乙腈.冰醋酸(25:62:10:3)为流动相;流速:1.0ml·min^-1;检测波长为212nmo柱温:30℃。结果芦丁在0.1639。0.9835峭范围内呈良好的线性关系(r=0.9998,n=6),平均回收率为99.5%.RSD为1.46%。结论该方法结果准确、重现性好,可用于测定绿梅止泻颗粒中芦丁的含量测定。
Objective To establish an HPLC method for the determination of rutin in Lumei Zhixie Granules. Methods Dalian Elite Hypersil ODS2 (200 mm ×4.6 mm,51μm)column was used. The mobile phase was methanol-water- acetonltrile-acetic acid (25: 62: 10: 3), the flow rate was:1.0 ml · min^-1 ;The detection wavelength was set at 212 nm ;and the column temperature was 30℃. Results The libration curve was linear in the range from 0. 163 9 μg to 0.983 5 μg (r = 0.999 8, n = 6), the average recovery was 99.5% with RSD was 1.46%. Conclusion The result is accurate and the reproducibility is good. This method can be used for the determination of Rutin in Lumei Zhixie Granules.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2006年第7期1184-1185,共2页
Lishizhen Medicine and Materia Medica Research