反相高效液相色谱法测定花芪通脉颗粒中灯盏乙素的含量

Determination of Scutellarin in Huaqi Tongmai Granula by RP-HPLC

目的建立测定花芪通脉颗粒中灯盏乙素含量的方法。方法采用反相高效液相色谱法RP—HPLC，ZORBAX-C18（4．6mm×150mm，5μm）色谱柱，甲醇-0.01mol·L^-1 磷酸缓冲液PH为5．2（35：65）为流动相，检测波长335nm，流速0．8ml·min^-1，枉温25℃。结果灯盏乙素在0．74—3．70μg范围内与峰面积呈良好线性关系，r=0．9996，灯盏乙素平均加样回收率为97．4％，RSD为0．98％（n=5）。结论：该方法可靠性高，准确性和重现性好，适用于花芪通脉颗粒中灯盏乙素的含量测定。

Objective To establish a simple and rapid reversed -phase high performance liquid chromatography method for the baseline separation, identification and assay of scutellarin in Huaqi Tongmai Granula. Methods The analytical column was a ZOD- BAX-CIs （4.6 mm × 150 mm,5 μm）filled with a 5 mm stationary phase. The mobile phase Consistent of methanol - （0.01 mol - L^-1 ）phosphate solution PH5.2（35：65）. The flow - rate was 0.8 ml·L^-1. The injection volume was 20 μl. The Detector wavelength was set at 335 nm, and the column temperature was 25℃. Results The calibration curve was linear over the range of 0.74 - 3.7 μg（ r = 0. 999 6）. The average recovery of the method was 97.4%, RSD was 0.98% （n = 5 ）. Conclusion The proposed method would be used in the quantitative analysis of scutellarin in Huaqi Tongmai Granula and the method is convenient,economical and accurate with good reproducibility.