高效液相色谱法同时测定杏香兔耳风中3种活性成分的含量

HPLC Determination of 3 Active Components in Ainsliaea fragrans Champ

目的建立同时测定杏香兔耳风中原儿茶酸、原儿茶醛和绿原酸含量的方法。方法采用反相高效液相色谱法．以ALLtech C18（4．6mm×250mm，5μm）为色谱柱，甲醇-1％冰醋酸水溶液（7：93）为流动相，检测波长280nm。流速：1．5ml·min^-1，柱温40℃。结果原儿茶酸、原儿茶醛和绿原酸的线性范围分别为0．02211—0．4422μg（r=0．9998），0．01501～0.2102μg（r=0．9999），0．09832—1．9664μg（r=0．9999）；回收率分别为98．39％（RSD=1．90％），97．89％（gSO=2．11％），98．27％（RSD=2．22％）。结论该法简便、准确、重现性好，适用于杏香兔耳风中3种成分的同时测定。

Objective An HPLC method was established to determine the contents of Ainsliaea flag＇ram Champ, 3,4-dihydroxybenzoic acid ,3,4-dihydroxybenzaldehyde and chlorogenic acid in Ainsliaeafiagrans Champ. Methods The samples were separated on a ALLtech C is column（4. 6 mm × 250 mm ,5 μm）with the mobile phase of methanol-1% acetic acid（ 7：93 ）. The flow rate was 1.5 ml · min^-1 , the column temperature was 40℃ and the detection wavelength was set at 280 nm. Results The linear ranges of 3, 4-dihydroxybenzoic acid ,3,4-dihydroxybenzaldehyde and chlorogenic acid were 0. 022 11 - 0. 442 2 μg （ r = 0. 999 9） ,0. 015 01 - 0. 210 2 μg （r = 0. 999 9） ,0. 098 32 - 1. 966 4 μg （r = 0. 999 9） respectively. The average recovery rate were 98.39% （ RSD = 1.90% ） ,97.89% （RSD = 2. 11% ） ,98.27% （RSD = 2.22% ） respectively. Conclusion The method is simple and quick with good reproducibility, it is suitable for the determination of the contents of 3 active components.