近交系小鼠微卫星位点的遗传标记多态性研究

Microsatellite DNA Polymorphisms in Inbred Strain Mice

目的研究近交系小鼠微卫星位点的遗传标记多态性及其意义。方法随机选取不同染色体的5个微卫星位点,应用PCR技术对BALB/C、豫医无毛小鼠、CBA、C57、DBA/2、C3H/HeJ、FVB/NJ及昆明小鼠共八种小鼠的DNA多态性进行研究。结果5对引物在近交系小鼠各基因座上均出现一条清晰的带,D16m it4、D3m it17、D3m it18具有多态性,D4m it2、D2Nds3不具有多态性。结论筛选出的引物D16m it4、D3m it17、D3m it18可用于检测近交系小鼠的品系来源和遗传背景,且所检测的小鼠符合近交要求。

Aim： To study the genetic marker polymorphisms and role of microsatellite of inbred strain mice. Methods ： Five microsatellites DNA loci on different chromosomes in eight kinds of strain mice including BALB/C .Yuyi hairless mice.CBA.C57 .DBA/2 .C3H/HeJ.FVB/NJ and Kunming mice were investigated by PCR analysis. Results ： all these mice tested display single allelic gene band with five pairs of primers, Polymorphism were found at D16mit4.D3mit17. D3mit18 loci, but not at D4mit2. D2Nds3 loci. Conclusion： Screened primers D16mit4. D3mitl7.D3mit18 showing marked polymorphisms could be used in determining the strains, origin and genetic background of mice, and these mice tested meet the reguest of inbred strain.