摘要
应用增殖细胞核抗原(PCNA)和P185单克隆抗体,通过免疫组织化学方法检测5例正常肾组织和45例肾癌组织中C-erbB-2癌基因产物P185蛋白和PCNA的表达状况。结果:5例正常肾组织中未发现P185蛋白和PCNA阳性表达;45例肾癌中P185蛋白和PCNA阳性表达率分别为84.4%和44.4%。表明阳性表达的P185蛋白和PCNA分别定位于肿瘤的细胞膜上和细胞核内;P185蛋白和PCNA阳性表达率与肾癌的病理分级、临床分期和患者术后生存期相关。提示P185蛋白和PCNA阳性表达在肾癌发生和发展中起着重要作用,可作为评价肾癌预后的新参数。
Expression of PCNA and P185 in 5 normal renal tissues and 45 renal cancer tissues were detected by immunohistochemical method. P185 and PCNA were located in membranate and nuclei respectively in renal cancer. The positive expression rates of P185 and PCNA for renal cancer were 38/45 and 20/45 respectively,but no staining was found in 5 normal renal tissues,and expression of P185 and PCNA related to the stage,grade and survival rates of renal cancer. It was suggest ed that expression of P185 and PCNA play an important role in the development of renal cancer,and may be a new indicator to assess the prognosis of renal cancer.
出处
《临床泌尿外科杂志》
北大核心
1996年第5期302-304,共3页
Journal of Clinical Urology