聚合酶链反应诊断小儿支原体肺炎及其意义

The Detection of Pediatric Mycoplasma Pneumonia Cases by Polymerase Chain Reaction and It's Clinical Significance

笔者用聚合酶链反应技术对70例小儿支原体肺炎鼻咽分泌物中肺炎支原体DNA进行检测。采用快速制备DNA模板的方法,简化了PCR实验过程,结果53例阳性,阳性率75.7%,而用支原体培养方法,阳性率仅53.8%(P<0.05),差异有显著性。对其中13例PCR阳性患者经红霉素静脉滴注治疗10～14天后,复测PCR,8例转阴性,5例仍为阳性。该方法既保持了PCR的敏感性和特异性,又简化过程,能够检测到相当于50个基因组DNA,可用于常规检测肺炎支原体,有助于支原体肺炎的早期诊断、早期对因治疗及疗效评价。

Mycoplasma DNA in nasopharynx secretion from seventy pediatric mycoplasma cases was detected, using rapid feit of PCR DNA sample preparation method and simple PCR processes. The result of PCR showed that: fifty-three positive cases with a positive rate 75.7%, comparing with only 53.8% positive rate using mycoplasma culture method (P<0.05). Thirteen PCR positive patients were treated by Venoclysis Erythromycin for 10-14 days but mycoplasma DNA was identified by PCR again. Among them, eight cases turned negative, but five cases still remained positive. The resalt showed that this method was not only more sensitive and reliable than conventional culture techniques, but also could simplify the PCR processes. It could be used for routine mycoplasma pneumonia test and for early diagnosis, which leads to early treatment and evaluation of therapeutic effect.