外源激素对欧洲白桦器官再生与继代培养的影响

Effect of Exogenous Hormones on Organogenesis and Subculturing of Betula pendula

欧洲白桦无性系2/86叶柄更容易诱导愈伤组织和不定芽的分化。6-BA的诱导效果好于ZT和TDZ,较适宜的培养基为MS+6-BA1.0+KT0.3+NAA0.01。6-BA质量浓度高与低均影响不定芽的诱导率。欧洲白桦不定芽和嫩茎的增殖过程可分为增殖分化阶段与壮苗阶段,通过控制培养基中细胞分裂素的质量浓度来完成对欧洲白桦试管苗生长状况的调控。

The clone 2/86 （Betula pendula Roth × Betula pendula var. carelica Sok） could be dedifferentiated into callus and further differentiate into buds using leafstalks as explants. Result showed that the inducing effect of 6-BA is better than that of ZT and TDZ. The better culture medium is MS macronutrients supplemented with 1.0 mg/L 6-BA ＋ 0.3 mg/L KT ＋ 0.01 mg/L NAA. The optimum concentration of 6-BA is 1.0 mg/L, concentration of 6-BA more than or less than 1.0 mg/L can influence the induction percentage of adventitious buds. The propagation process of birch adventitious buds and shoot tips is divided into two stages： multiplication stage and fast-growing stage. Moreover, the level of cytokinin in the medium can be regulated to ensure that shoots grow healthy and strong.