肝细胞癌凋亡病变的原位末端标记显示

In Situ end labeling detectin for apoptosis in hepatocellular carcinoma

原位末端标记（ＩＳＥＬ）是显示凋亡的新技术。按Ｇａｖｒｉｅｌｉ法对４６例未接受过化疗与放疗的人肝细胞癌（ＨＣＣ）作回顾性（ＩＳＥＬ）研究，结果显示在对照系列中阴性与阳性均与理论相符，４６例ＨＣＣＩＳＥＬ（＋）可分为：①单个细胞（＋）；②点状（＋）；③小片状（＋）；④大片（＋）．Ⅰ级ＨＣＣ以单细胞（＋）较多；Ⅱ、Ⅲ级以小片状（＋）较多（Ｐ＜０．０５）．单个细胞（＋）为凋亡经典概念，而片状（＋）报道还不多。本文小片状（＋）很普遍，常位于癌巢中央，其ＨＥ形态以核浓缩为主，此结果表明，通常ＨＣＣ所见小片状＂变性坏死样改变＂可能为凋亡而非坏死。

cases of hepatocellular carcinoma were retrospectively studied by in situ end labeling(ISEL)method according to Gavrieli's.All patients had not been treated with chemotherapy or radiotherapy.The results revealed that:①Negative and positive control series were in accordence with what they were expected.②Positive patterns of cancer slides were:single cell(+),dot-like(+),small piece(+)and massive(+).The 'small piece (+)'were frequently seen in the center of the cancer nests;they were'necrobiosis-like'in HE stain with more pyknosis.It was considered as apoptosis but not necrosis in nature.