胶质瘤Polo样激酶1的表达与肿瘤细胞增殖活性及预后的关系

PLK1 Expression in Gliomas and Its Correlation to Tumor Proliferative Activity and Prognosis

目的探讨Polo样激酶1(PLK1)在人脑胶质瘤组织上的表达及其与胶质瘤细胞增殖活性、临床病理和预后的关系。方法应用免疫组化法检测47例胶质瘤和8例正常脑组织中的PLK1和增殖细胞核抗原(PCNA)的表达,并随访病人。结果正常脑组织未见PLK1明显表达。Ⅰ～Ⅱ级、Ⅲ级和Ⅳ级脑胶质瘤中PLK1表达阳性率分别为64.3%、93.8%和100%,Ⅲ和Ⅳ级分别与Ⅰ～Ⅱ级比较差异有显著性意义(P<0.05)。PLK1表达与胶质瘤病理级别和PCNA表达均呈正相关(r=0.424,P<0.05;r=0.745,P<0.01)。PLK1高表达组和低表达组患者两年生存率分别为41.4%(12/29)和72.2%(13/18),差异显著(P<0.05)。结论PLK1在胶质瘤的发生发展和增殖中起重要作用,与肿瘤分化程度和患者预后密切相关。

Objective To study the expression of polo-like kinase-1 （PLK1） in glioma and its correlation to tumor proliferative activity and patiens＇ prognosis. Methods The expression of PLK1 and proliferative cell nuclear antigen （PCNA） in 8 cases of normal brain tissues and 47 cases of gliomas were detected by immunohistochemical staining. All the patiens were followed up. Results No expression of PLK1 was found in the normal brain tissues. The positive rate of PLK1 expression in gliomas of grade Ⅰ - Ⅱ （64.3%） was significantly lower （P〈0.05） than that in gliomas of grade Ⅲ （93.8%） and grade Ⅳ （100%）. The expression of PLK1 in glioma was significantly associated positively with pathological grade and PCNA labelling index （r=0.424, P〈0.05; r=0.745, P〈0.01, respectively）. Two years survival rate of the patients with high PLK1 expression （41.4%） was significantly lower （P〈0.05） than that of patients with low PLK1 expression （72.2%）. Condusions The high expression of PLK1 may play an important role in the pathogenesis and development of gliomas. The expression of PLK1 is closely related to the pathological grade of the tumors and prognosis in the patients with gliomas.