摘要
目的:探讨survivin基因在化疗药物三氧化二砷(As2O3)和顺铂(DDP)诱导的喉鳞癌(Hep-2)细胞凋亡中的作用。方法:用Hep-2细胞株进行培养传代,以不同浓度的As2O3和DDP作用于Hep-2细胞,MTT观察As2O3和DPP对Hep-2细胞生长的抑制情况,TUNEL法检测细胞凋亡数量,用RT-PCR方法检测survivin基因的表达。结果:As2O3和DDP对Hep-2细胞的生长均有抑制作用,具有时间-剂量依赖性,化疗药物组细胞凋亡率和survivin基因表达抑制率高于对照组。结论:在As2O3和DPP诱导的Hep-2细胞凋亡中survivin基因表达被抑制,survivin基因可能在DPP和As2O3抗肿瘤中发挥重要作用。
Objective： To study the role of survivin gene on the apoptosis of the laryngeal squamous cell carcinoma Hep - 2 strain induced by arsenic trioxide （As2O3） and cisplatin （DDP）. Methods：The laryngeal squamous cell carcinoma nep - 2 strain was chosen in this experiment, The inhibitory effects of clemotherapeutical drugs on Hep - 2 strain were assayed with MTT test. Apoptosis was detected by TUNEL test. Expression of survivin gene was detected by reverse transcriptase PCR. Results： Both As2O3 and DDP obviously inhibited the laryngeal squamous cell carcinoma Hep - 2 cell growth with dose and time - dependent. Apoptosis and inhibitory rate of survivin gene expression induced by As2O3 and DDP was higher than that in control group. Conclusions： The survivin gene expression was inhibited during the course of apoptosis in the laryngeal squamous cell carcinoma Hep - 2 strain induced by As2O3 and DDP. The survivin gene may play a critical role on the inhibiting carcinoma of As2O3 and DDP.
出处
《现代生物医学进展》
CAS
2006年第6期13-15,F0003,共4页
Progress in Modern Biomedicine
基金
黑龙江省卫生厅科研课题资助
编号2004-133
