用原子力显微镜研究炎症反应中粘附分子间相互作用

Measuring Molecule Adhesion Forces In Inflammatory Processes with Atomic Force Microscope(AFM)

在炎症反应中,白细胞在血液流动动力和粘附分子的作用下在血管内皮上的滚动,是白细胞从流动的血液中浸润、迁移到炎症部位整个过程的第一步。白细胞在内皮细胞上滚动由选择素分子与其配体分子相互作用所导致,其中P选择素分子(P-selectin)与其对应的P-选择素糖蛋白配体-1(PSGL-1)的相互作用起着重要的作用。原子力显微镜技术能定量分析P-se-lectin/PSGL-1相互作用的动力学反应。

Leukocytes roll along the endothelium of postcapillary venulae in response to inflammatory and thrombotic processes, which is, under hydrodynamic shear forces, the first step in directing leukocytes out of the blood stream into sites of inflammation, and mainly resulted from the interaction between P - selectin on the endothelial cell membrane and P - selectin glycoprotein ligand - 1 （PSGL - 1 ） on the leucocyte microvilli. Kinetics of P- selectin/FSGL- 1 interaction was made a quantitative analysis by using atomic force microscope （AFM）.