表皮生长因子和降钙素基因相关肽在高频电磁场治疗急性胃损伤中作用研究

Study of Role of EGF and CGRP in Exposure of HEMFs in Treating Acute Gastric Mucosal Injuries in Rats

目的:探讨高频电磁场(high-frequency electromagnetic fields,HEMFs)曝露治疗急性胃损伤的作用机制。方法:选用健康SD大鼠,以Indomethacin灌胃法复制胃粘膜急性损伤模型,观察40.68MHz HEMFs(波长为7.3m)曝露(微热量,30-50mA,15min,1/d)治疗1或6次后,大鼠胃粘膜损伤程度(胃损伤指数和病理损伤积分)、胃粘膜血流量、血浆表皮生长因子(epidermalgrowth factor,EGF)和降钙素基因相关肽(calcitonin gene-related peptide,CGRP)水平。结果:HEMFs曝露1次后,胃粘膜血流量较对照组明显升高(p<0.05),大鼠胃粘膜损伤程度、血浆EGF和CGRP水平较对照组均无显著性差异(p均>0.05);HEMFs曝露6次后,胃粘膜损伤程度较对照组明显改善(p均<0.05),胃粘膜血流量显著升高(p<0.05),血浆EGF和CGRP水平较对照组无显著性差异(p均>0.05)。结论:HEMFs曝露治疗大鼠胃粘膜急性损伤可能与其增加胃粘膜血流量有关,与血EGF和CGRP无关。

Objective： To elucidate the treatment mechanism of the exposure of high - frequency electromagnetic fields（HEMFs） to acute gastric mucosal injuries in rats. Methods： The models of acute gastric mucosal injuries were replaced with the gastricgavage of indomethacin in healthy SD rats. The rats in experiment groups were exposed to 40.68MHz HEMFs（30 - 50mA） for 15 minutes, 1/d, while rats in control groups were exposed to HEMFs without electric current output. After exposed to HEMFs once or for six times, the degree of gastric mucosal injury（ injury index and integral of pathological diagnosis）, gastric mucosal blood flow（GMBF）, and the levels of epidermal growth factor（EGF） and calcitonin gene - related paptide（CGRP） in plasma were observed. Results： After exposed to HEMFs once,the level of the rats＇ GMBF in experiment group was much higher than that in control group（p 〈 0.05）, the degree of gastric mucosal injuries（DGMI） and the levels of EGF and CGRP in plasma in experiment group showed no significant difference from those in control group（ p all 〉 0.05）. After exposed to HEMFs for six times, the degree of gastric mucosal injuries was significantly reduced（ p all 〈 0.05）, and GMBF increased（p 〈 0.05）, but there was no marked difference in tire levels of EGF and CGRP between experiment and control groups（ p all 〉 0.05）. Conclusion： The therapeutic mechanism of HEMFs on acute gastric mucosal injuries in rats is related to the increase of GMBF, not to EGF and CGRP in plasma.