内皮抑素对人脐静脉内皮细胞(HUVEC)及体外微血管模型的作用及其可能的机制

The inhibitory mechanism of endostatin on human umbilical vein endothelial cells(HUVEC)

目的：探讨内皮抑素对人脐静脉内皮细胞（HUVEC）及体外微血管模型的作用及其可能的机制。方法：1．MTT法检测不同浓度（10～50μg／ml）内皮抑素作用72h和30μg／ml内皮抑素作用不同时间（24～72h）对HUVEC细胞的影响；2、电镜观察HUVEC细胞超微结构的变化；3．光镜下观察内皮抑素（30μg／ml）对体外人造血管模型的影响。结果：1．MTT检测显示，内皮抑素（20～50μg／ml）能抑制HUVEC细胞的增殖（P〈0．05，P〈0．01），具有剂量-时间依赖性。2．电镜观察，HUVEC细胞内皮抑素作用组均出现凋亡改变。3．光镜观察，内皮抑素能抑制新生血管的形成，并能破坏新生的血管网。结论：内皮抑素能抑制人脐静脉血管内皮细胞HUVEC的增殖，并具有时间一剂量依赖性，机制可能为诱导细胞凋亡。提示，内皮抑素可能通过诱导HUVEC的凋亡抑制其增殖，并能破坏新生的血管。内皮抑素可能以此抑制机体肿瘤的生长与转移。

Objective： To evaluate the inhibitory effect and the possible mechanism of endostatin on human umbilical vein endothelial cells （HUVEC）. Methods： By means of MTT assay, the influences of endostatin with different concentrations（ 10- 50μg/ml） and endostatin （30μg/ml） with different time（24- 72h） on the HUVEC cells were studied. The changes of HUVEC cells＇ ultrastructures were examined by electron microscope. The influences of endostatin（30μg/ml） on the ectogenetic artificial blood vessel models were observed by photic microscope. Result： The growth of HUVEC cells was significantly inhibited by endostatin（20- 50μg/ml） （P〈 0.05, P〈 0.01）, with dose- time- dependence. Apoptosis was observed in the groups of HUVEC cells with endostatin by electron microscope. Endostatin could not only inhibit the formation of neonatal blood vessels but also destroy the formation of neonatal blood vessel nets. Conclusion： Endostatin can inhibit the proliferation of HUVEC cells with time - dose - dependence and it＇ s mechanism maybe induces the apoptosis. It is pointed out that Endestatin can inhibit the proliferation of HUVEC cells, probably inducing the apoptosis, which can destory the neonatal blood vessels. Endostatin perhaps inhibits the growth and the transformation of human carcinoma according to tiffs mechanism.