香樟未成熟合子胚体胚发生及植株再生研究

Study on Somatic Embryogenesis and Plant Regeneration of Immature Zygotic Embryo of Cinnamomum camphora

以香樟的未成熟幼胚为外植体进行诱导体胚发生影响因素的研究。成功地从香樟幼胚中诱导出胚状体并获得再生植株,MS为基本培养基,蔗糖是最佳的碳源。经2年对初生胚的继代培养,获得胚性愈伤组织;胚性愈伤组织经体胚诱导、体胚成熟、体胚萌发3个过程的诱导后能够获得再生植株。

Somatic embryogenesis was successfully induced from immature embryos of Cinnamomum camphora. The best carbohydrate form was sucrose under the condition of MS ＋ 1.0 mg· L^-1 BA ＋ 0.1 mg· L^-1 2,4-D ＋ 700 mg· L^-1 CH. After subculture of 2 years, embryogenic calli were observed on the somatic embryos. Embryogenic calli were transferred onto induction medium for somatic embryos initiation, maturation medium for somatic embryo maturing, and then to germination medium for shoot induction. The embryogenic calli could be induced to develop into plantlet and successfully transferred to outdoor environment.