摘要
为研究大豆磷脂酰胆碱的色谱分离过程,建立了考虑轴向扩散、液膜传质阻力和内扩散阻力的一般性速率模型,用有限元和正交配置法求解该模型.模拟结果与实验数据吻合,并得到了液膜传质系数与流速的关系及孔内有效扩散系数.采用乙醇-水体系分离了大豆浓缩磷脂中的磷脂酰胆碱,考察了流动相组成和流速对分离效果的影响.随着流动相中水质量分数的增加,磷脂酰胆碱的容量因子和分离度减小.流动相中的水质量分数以20%为最佳.
A general rate model considering axial dispersion, liquid film mass transfer and effective diffusion in particle was adopted to investigate the chromatography process for soybean phosphatidylcholine. Finite element and orthogonal collocation methods were used to transfer these equations to ordinary differential equations and to solve them. The simulation results agreed with experimental data very well. The relationship between the film mass-transfer coefficient and the interstitial velocity and the effective diffusion coefficient were measured. Total phosphatidylcholine was isolated from soybean lipids with ethanolwater. The effect of composition and flow rate of mobile phase was investigated. As the water concentration o-f mobile phase increased,the capacity factor and the resolution decreased. The best water concentration of mobile phase was 20 %.
出处
《浙江大学学报（工学版）》
EI
CAS
CSCD
北大核心
2006年第7期1267-1271,共5页
Journal of Zhejiang University：Engineering Science
关键词
液相色谱
一般性速率模型
大豆卵磷脂
分离
liquid chromatography
general rate model
soybean phosphatidylcholine
separation
