癌组织中幽门螺杆菌球形体的超微结构研究(英文)

Ultrastructural study on the coccoid forms of Helicobacter pylori in the tissues of carcinoma

目的揭示癌组织中幽门螺杆菌(Hp)球形体的超微结构特征。方法通过透射电镜观察Hp球形体感染阳性的4例胃癌、2例食管癌组织中Hp球形体的超微结构。结果Hp球形体多散在性分布于癌巢、癌间质以及进入癌细胞、巨噬细胞等细胞胞质。Hp球形体与其他细菌的L型样,形态不规则,呈多形性,且大小不等、胞质电子密度不等、胞壁缺失等。Hp球形体有A、B两种类型,A种体积较小,胞浆致密,细胞膜上带有鞭毛,此种活性较强;B种体积较大,胞浆疏松,细胞膜上不带鞭毛,此种可能已蜕变结论Hp球形体仍具有活性,在Hp的传播、相关疾病的治疗复发和相关肿瘤的发生过程中起重要的作用。

Objective：This study was designed to characterize the ultrastructure of the coccoid Helicobacter pylori（ H. pylori） forms in the tissues of carcinoma. Methods： Transmission electron microscopy （ TEM ） technique was used to examine the structure of coccoid H. pylori in 4 cases of gastric carcinoma and 2 cases of esophageal carcinoma. Results： It was found that the coccoid H. pylori were scattered in cancer nest and cancer interspace, some of which enterded cancer cells, macrophages and other cells. The coccoid H. pylori appeared to be similar to the L-forms of other bacteria with varying cell shape and size, different cytoplasmic electron densities and defects in cell wall. Two types of coccoid H. pylori could be distinguished in the tissues of carcinoma by TEM. Type A was smaller with higher electron density in the cytoplasma, and flagellation on the cell membrane, suggesting that it could be viable. However, the type B was larger with lower electron density in the cytoplasma, and no flagellation on the cell membrane, suggesting that it could be a degenerative form. Conclusions ：The coccoid forms of H. pulori still have certain degree of viability, thus they may play an important role in the transmission of H. pylori,the relapse of H. pylori related disease after treatment and the development of the related carcinoma.