脉冲电场对中国红豆杉细胞的影响

Effect of Pulsed Electric Field on the Suspension Cultures of Taxus chinensis

通过二维电泳(2-DE)技术来研究脉冲电场作用后48h中国红豆杉细胞在蛋白表达上的变化．共进行了3次二维电泳重复实验,经过相关因子分析证实具有较高的重复性．比较对照组和经过脉冲电场处理的实验组的二维电泳图谱,发现有较明显的差异．用基质辅助激光解析-飞行时间质谱和液相色谱-电喷雾质谱对其中的部分蛋白进行分析鉴定．鉴定结果表明：核糖核酸蛋白粒、醛糖差向异构酶和铁氧还蛋白-NADP(+)还原酶这三个蛋白可能是与脉冲电场诱导相关的蛋白．

Two dimensional gel electrophoresis（2-DE） was used to analyse changes of the protein expression of suspension cultures of Taxus chinensis 48 hours after pulsed electric field （PEF） elicitation. The 2-DE experiments were repeated for 3 times, and proved to be reliable according to the correlation coefficient analysis. Distinct changes were found from comparison of the protein patterns of control and PEF treated cells. Matrix-assisted laser desorption/ionization mass spectrometry and electrospray ionization mass spectrometry were used to analyse some proteins. The results indicated that heterogeneous nuclear ribonucleoprotein（hnRNP）, aldose l-epimerase family protein and Ferredoxin-NADP（ ＋ ） reductase, putative/adrenodoxin reductase are related to the induction of PEF.