粉纹夜蛾5BI细胞的悬浮培养

THE SUSPENSION CULTURE OF 5BI CELL LINE FROM THICHOPUSIA NI BY SPINNER FLASK

报道了粉纹夜蛾５ＢＩ细胞在转瓶系统悬浮培养及血清浓度、细胞接种浓度、非离子表面活性剂ＰｌｕｒｎｉｃＦ－６８等对细胞生长的影响与作用．实验结果显示：５ＢＩ细胞能够在搅拌条件下，在含５％血清培养基中正常生长增殖，当血清浓度降至２％时，细胞生长速率产生较明显变化；ＰｌｕｒｏｎｉｃＦ－６８对细胞生长有显著的保护作用．

The suspesion culture of 5BI cell line from Thichoplusia ni and culture conditions effcted on cell growth including serum concentration, seeding density and nonionic surfactant pluronic F 68 were studied in this paper. It was demonstrated that 5BI cell could normally grow and propagate under agitating condifion. Cell growth rate decreased as serum concentration was reduced to 2%. pluronic F 68 was responsible for protection of 5BI insect cells from advers effects of agitating.