高效液相色谱──高灵敏催化光度法测定钛

Highly Sensitive Catalytic Determination of Ti with HPLC Detection

在１００℃稀硝酸介质中及活化剂联吡啶存在条件下，痕量钛（Ⅳ）对过氧化氢氧化甲基橙褪色这一缓慢反应具有很强的催化作用。用自来水冷却中止反应，采用高效液相色谱检测甲基橙浓度的变化，建立了一个测定０．８ｎｇ／ｍＬ～６０ｎｇ／ｍＬ钛的高效液相色谱新方法，并用于试样分析。

Under the conditions of 0. 03 mol/L HNO3-5. 0×10-4 mol/L 2, 2'-bipyridine (as an activator) at 100℃, Ti (IV) had strong catalytic effect on the decolour reaction between methyl orange and H2O2. The UV-detector for HPLC was chosen to monitor the changes in concentration of methyl orange in the reaction system.A novel catalytic reaction-HPLC method for the determination of 0. 8ng/mL￣60ng/mL Ti was described by the fixed-reaction time procedure.Titanium in human hair samples was analysed with good results.