缺铁性贫血大鼠模型建立和补充乳酸亚铁改善血红蛋白水平研究

Establishment of Rat Model of Iron Deficiency Anemia(IDA) and Improvement of Iron Status with Supplement of Ferrous Lactate

为探讨缺铁性贫血大鼠模型建立及补充乳酸亚铁后大鼠生长发育和血红蛋白水平的变化,为相关研究提供参考数据,采用二级初断乳Wistar大鼠25只,体重50～60g,在二级动物房单笼饲低铁饲料4周,测定尾血血红蛋白(Hb),将Hb低于100gL的大鼠依血红蛋白水平随机分成缺铁对照组和补充铁组,每组10只。实验期为4周。缺铁大鼠和乳酸亚铁补充组大鼠的及其差值均有差异有非常显著性(P<0.0001);缺铁性贫血大鼠肝脏重量和心脏重量低于补充乳酸亚铁组大鼠(P<0.0002),而脾脏和肾脏重量高于补充乳酸亚铁组大鼠,两组脾脏重量差异有显著性(P<0.05);缺铁性贫血大鼠的脾脏体重与心脏体重显著大于补充乳酸亚铁组大鼠(P<0.05),补充乳酸亚铁组大鼠的肾脏体重则大于缺铁性贫血大鼠(P=0.001);缺铁对照组大鼠的血红蛋白含量非常显著低于补充乳酸亚铁组大鼠(P<0.0001),而缺铁对照组大鼠的红细胞游离原卟啉含量非常显著高于补充乳酸亚铁组大鼠(P<0.0001),缺铁组大鼠的白细胞数量是补充组的3.4倍,红细胞数量却仅为补充组的33.9%。缺铁大鼠模型建立需要严格控制饲养条件,缺铁大鼠通过一定的代偿性增生改善机体适应缺铁和缺氧状况的能力有限,补充乳酸亚铁后可以有效地改善缺铁状况。

To explore the establishment of rat model of iron deficiency anemia and observe the change of hemoglobin （Hb） level after supplement of ferrous lactate, 25 SPF weanling female Wistar rats weighing 50 - 60 g were fed low iron diet for 4 weeks, and 20 of them with hemoglobin less than 100 g/L were randomly divided into 2 groups （ control group and supplement group）. The duration of this experiment was 4 weeks. At the end of the experiment, the body weight of supplement group was higher than that of control group （P 〈 0.001）. The liver and heart weights of control group were lower than those of supplement group （P 〈 0.01 ）. The spleen weight of control group was higher than supplement group （ P 〈 0.05）. Hemoglobin （Hb） level of control group was lower than that of supplement group （P 〈 0.001 ）. The free erythrocyte porphyrin （FEP） of control group was higher than that of supplement group （P 〈 0.001 ）. WBC counts of control group were 3.4 times those of supplement group and RBC counts were 33.9% those of supplement group. The IDA model was successful established with strict control of the condition, and the supplement of ferrous lactate to rats with IDA improved the Hb level.