固相微萃取-气相色谱/质谱测定植物叶片中的挥发性物质

Analysis of Volatile Constituents from Leaves of Plants by Gas Chromatography/Mass Spectrometry with Solid-Phase Microextraction

采用固相微萃取(SPME)方法吸附植物叶片中的挥发性物质,然后采用气相色谱/质谱法(GC/M S)分析了挥发性物质的成分。在45℃水浴温度下,采用Po lyacry la te(85μm)固相微萃取头,在广口瓶中植物叶片的上方顶空吸附60m in,然后进行GC/M S分析。结果表明,植物叶片中的挥发性物质得到了很好的分离,受山楂叶螨(Tet-raychu s vienn eis)危害严重的植物的完好叶片中的挥发性物质均含有顺-3-己烯-1-醇乙酸酯、顺-3-己烯-1-醇丁酸酯和α-法呢烯,且含量较大。初步确定这些物质是对山楂叶螨具有引诱作用的主要物质,从而为利用天然生物活性物质防治山楂叶螨提供了理论依据。

A method for analyzing volatile constituents from plant leaves with gas chromatography/mass spectrometry （GC/MS） coupled with solid-phase microextraction （SPME） was developed. The volatile compounds from the plant leaves inside a sealed flask maintained at 45 ℃ in a water bath were efficiently extracted with Polyacrylate （85 μm） SPME fibers prior to perform GC/MS analysis. The GC/MS analysis indicated that the volatile compounds from the plant leaves which is easy to be damaged by Tetraychus vienneis include relative large amounts of cis-3-hexenyl ester acetic acid, cis-3-hexenyl ester butanoic acid and alpha-famesene. These compounds were preliminarily confirmed to be accountable for attracting Tetraychus vienneis. This finding may lead to identify biological species for preventing and treating Tetraychus vienneis.