海洛因对雌性大鼠催乳素基因表达的影响

Effect of heroin on prolactin gene expression in female rats

目的:研究海洛因对雌性大鼠垂体催乳素(PRL)基因表达的影响。方法:50只雌性Wistar大鼠,随机分为5组(每组10只):对照组、海洛因给药3和9 d组、海洛因给药9 d后停药3和9 d组;逆转录聚合酶链反应(RT-PCR)检测垂体PRL mRNA相对含量,放射免疫分析法(RIA)检测血浆催乳素(PRL)水平。结果:给药3和9 d组及停药3 d组大鼠垂体PRL mRNA水平显著低于对照组(P<0.01),停药9 d组PRL mRNA的水平虽低于对照组,但差异无显著性(P>0.05);与给药9 d组相比,停药3 d组PRL mRNA水平有增高趋势,但差异无显著性(P>0.05),停药9 d组的PRL mRNA的水平则显著高于给药9 d组(P<0.05)。给药3和9 d组及停药3和9 d组血浆PRL显著低于对照组(P<0.01);与给药9 d组比较,停药3 d组的血浆PRL水平仍然较低(P<0.05),停药9 d组的PRL水平有高于给药9 d组的趋势,但差异无显著性(P>0.05)。结论:海洛因使雌性大鼠垂体PRL mRNA表达水平下降,短期停用海洛因其无法恢复到正常水平。

Objective To investigate effects of heroin on prolactin （PRL） mRNA in female rats. Methods Fifty female Wistar rats were randomly divided into five groups （10 rats each group） ： control, heroin-treated-3 d, heroin- treated-9 d, heroin-withdrawal-3 d and heroin-withdrawal-9 d groups. PRL mRNA of pituitary was assessed by RT-PCR and PRL concentration in rat plasma was determined by radioimmunoassay （RIA）. Results The expression levels of PRL mRNA of pituitary were declined significantly in heroin-treated-3 d, 9 d and heroinwithdrawal-3 d groups compared with control group （P〈0. 01）. Compared with heroin-treated-9 d group, PRL mRNA level in heroin-withdrawal-9 d group was increased significantly （P〈0.05）. Compared with control group, the PRL level was lower significantly in heroin-treated-3 d and 9 d groups （P〈0.01） ; Also, the PRL levels were decreased significantly in heroin-withdrawal-3 d and 9 d groups （P 〈 0.01）. The PRL level was decreased remarkably in heroin-withdrawal-3 d group compared with heroin-treated-9 d group （P 〈 0.05）; in heroin- withdrawal-9 d group, the level of PRL was higher than that in heroin-treated-9 d group, but the difference was not significant （P〉0.05）. Conclusion The PRL mRNA in the pituitary and the PRL concentration in plasma in female rats are decreased by heroin and the effect can not be reversed in a short term after heroin withdrawal.