抗衰老口服液对自然衰老大鼠自由基的影响

Effect of anti-aging oral preparation on free radical in senescent rats

目的:探讨中草药复方制剂抗衰老口服液(AOP)对自然衰老大鼠自由基的清除作用及其机制。方法:健康自然衰老Wistar大鼠(22月龄)40只,分为5组:对照组,AOP低、中、高剂量组,青春宝抗衰老片(QCB)组。灌胃给药,每天1次,30 d后大鼠断头处死,取血,分离血浆;取心肌组织和脑组织,液氮冻存。检测血浆超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、丙二醛(MDA)、一氧化氮(NO),脑组织腺苷三磷酸酶(ATPE)以及心肌脂褐素的含量。结果:AOP中、高剂量组和QCB组大鼠血浆SOD含量、CAT含量、大脑皮层中ATP酶的含量均高于对照组(P<0.05);血浆NO含量、MDA含量、心肌脂褐质含量均低于对照组(P<0.05)。结论:中、高剂量AOP可减少自然衰老大鼠血浆自由基的产生、加速自由基的清除,促进脑组织能量代谢,具有抗衰老的生物学作用。

Objective To investigate the effect of anti-aging oral preparation （AOP） on free radical in senescent rats. Methods The natural senescent and healthy rats were randomly divided into five groups., control group, low , middle and high doses of AOP groups and Qingchunbao group. Drugs were given by perfusing stomach once a day for 30 d successively. At the 30th day the rats were killed by breaking heads. The blood was collected and the plasma was separated, the brain and myocardium were collected, all of them were frozen in the liquid nitrogen. Then the plasma contents of SOD, CAT, MDA, NO, ATP of brain, lipofuscin of myocardium in the rats were detected. Results The plasma contents of SOD, CAT and ATPase of cerebral cortex in middle, high doses of AOP group and Qingchunbao group were higher than those in control group （P〈0. 05） , and the plasma contents of NO, MDA, lipofuscin of myocardium were lower than those in control group （P〈0. 05）. Conclusion Middle and high doses of AOP can reduce the development of the free radical and accelerate the clearance of the free radical, facilitate energy metabolism of the brain, and AOP has the anti-aging effect.