化疗药物对舌癌细胞增殖能力的影响

Effects of chemotherapeutic drugs on proliferation of tongue carcinoma cells

目的:观察紫杉醇、丝裂霉素、5-氟尿嘧啶、顺铂4种化疗药物对舌鳞状细胞癌细胞增殖能力的影响,以指导临床合理用药,减少药物的副作用。方法:收集手术切除的舌癌组织,制备单细胞悬液进行培养。选择紫杉醇、丝裂霉素、5-氟尿嘧啶、顺铂4种化疗药物进行药物敏感性实验,以不加药物为对照组,通过MTT比色法和流式细胞术法观察舌癌细胞增殖抑制率和细胞周期各时相的变化。结果:紫杉醇、丝裂霉素、5-氟尿嘧啶对舌癌细胞增殖抑制率分别为45.3%、37.3%和36.2%,明显高于对照组(2.1%)(P<0.01);顺铂与对照组比较差异无显著性。流式细胞术测定结果显示,紫杉醇、丝裂霉素、5-氟尿嘧啶组与对照组比较,其G0/G1期细胞增多,S期细胞减少,G2/M期细胞相对增多(P<0.01)。结论:紫杉醇、丝裂霉素和5-氟尿嘧啶能明显抑制舌癌细胞的生长,临床上应优先考虑使用。

Objective To observe the effects of paclitaxel, mitomycin, 5-FU and cisplatin on the proliferation of tongue carcinoma cells and to guide the reasonable using of chemotherapeutic drugs and avoids the side-effects. Methods The fresh tongue carcinoma tissues from surgery were collected and unicellular suspension were prepared. Then paclitaxel, mitomycin, 5-FU and cisplatin were used to investigate the drug sensitivity on primary tongue carcinoma cells and a blank control group was set up. The inhibitory effects of chemotherapeutic drugs on the growth of tongue carcinoma cells and the cell cycle phases were observed by MTT colorimetry and flow cytometry. Results The inhibitory rates of paclitaxel, mitomycin and 5-FU on tongue carcinoma cell proliferation were 45.3%, 37. 3%, and 36.2%, respectively, and in control group it was 2.1%, the difference was significant （P〈0. 01）. But there was no significant different of inhibitory rate between cisplatin and control group. The results of flow cytometry showed that the amount of cells increased in G0/G1 phase, decreased in S phase and relatively increased in G2/M phase after using paclitaxel, mitomycin and 5-FU compared with control group （P 〈 0.01）. Conclusion Paclitaxel, mitomycin and 5-FU can inhibit the growth of tongue carcinoma cells distinctively and should be considered prior to other drugs.