还原型谷胱甘肽对六价铬诱导的L-02肝细胞毒性的保护作用

Protective effect of reduced glutathione on cytotoxicity induced by hexavalent chromium[Cr(VI)] in L-02 hepatocyte

目的探讨还原型谷胱甘肽(GSH)对六价铬[Cr(Ⅵ)]诱导的L-02肝细胞毒性的影响。方法分别设Cr(Ⅵ)、GSH单独作用组、联合作用组和空白对照组,采用MTT法检测细胞生存率的变化。结果在2、4、8、16、32和64μmol/LCr(Ⅵ)处理浓度,Cr(Ⅵ)对L-02肝细胞具有明显细胞毒性,细胞存活率与Cr(Ⅵ)处理浓度呈负相关(r=-0·910)。仅浓度为20μmol/L的GSH对Cr(Ⅵ)诱导的细胞毒性具有拮抗作用,Cr(Ⅵ)+GSH联合组的生存率与Cr(Ⅵ)不同浓度单独处理组比较差异均具有显著性(P<0·05)。结论适宜浓度的GSH可能对Cr(Ⅵ)诱导的L-02肝细胞毒性具有保护作用。

Objective To explore the effect of reduced glutathione （ GSH ）on cytotoxicity induced by hexavalent chromium（ Cr（ Ⅵ ） ） in L-02 hepatocyte. Methods The test has three groups ： the groups of （Cr（ Ⅵ ）, the groups of GSH, the groups of Cr（Ⅵ）and GSH. The survival rate of L-02 hepatocyte is assessed on the reductions of tetrazolium dye （MTT）. Results Significant cytotoxicities of L-02 hepatocyte were observed at the concenations of 2,4,8,16,32 and 64μmol/L Cr （Ⅵ）. Concentration-dependent decrease in cell survival rate of Cr （Ⅵ）-treated L-02 hepatocytes were observed（ r = -0.910）Protective effect on all concentrations of Cr（Ⅵ）（2- 64μmol/L） at the dose of 20μmol/LGSH were found. Conclusion The results demonstrated that proper concentrations of GSH could have protective effect on cytoxicity induced by Cr（Ⅵ） in L-02 hepatocyte. GSH of too low or too high concentrations don＇t has this effect.