胆固醇酯转运蛋白基因多态性与肥胖及对膳食干预的影响

Association of gene polymorphism at cholesterol ester transfer protein locus with obesity and response to dietary intervention in obesity

目的探讨胆固醇酯转运蛋白(CETP)基因多态性与肥胖的关系及其对肥胖膳食干预的影响。方法从血凝块中提取DNA,用聚合酶链反应和限制性片段长度多态性方法(PCR-RFLP)检测上海340名成年人的CETP基因TaqIB位点多态性;对研究对象进行体格检查并测定血脂;对其中的肥胖人群进行膳食干预,分析CETP基因多态性对干预效果的影响。结果(1)B1B1、B1B2和B2B2三种基因型的频率分别为35·6%、47·9%和16·5%,符合Hardy-Weinberg定律;B1等位基因为优势等位基因;肥胖组人群和正常组的基因型构成差异无显著性,控制相关影响因素后结果相同。(2)三种基因型的高密度脂蛋白(HDL)水平差异有显著性,B2B2型的HDL水平最高。控制环境影响因素后这种相关性仍然存在。(3)B1B2型膳食干预后HDL水平明显升高,这与其他基因型显著不同;控制基线HDL水平和性别因素后基因型对HDL水平改变没有影响。结论CETP-TaqIB位点B2B2基因型具有较高的血清HDL水平,成年肥胖人中此位点多态性无特殊性;基线HDL水平影响不同基因型的HDL对膳食干预的反应。

Objective To study the association of gene polymorphism at cholesterol ester transfer protein（CETP） locus with obesity and response to dietary intervention in obesity. Methods The PCR-PFLP method was used to detect the polymorphism of CETP gene of 340 adults in Shanghai. The levels of serum lipid profile, including TG, TC, HDL and LDL were analyzed. Obesity was selected to dietary intervention. Results （ 1 ） The genotype frequencies of CETP-TaqIB B1B1 , B1B2 and B2B2 were 35.6%, 47.9% and 16,5% respectively, which were in agreement with Hardy-Weinberg equilibrium. There was no significant difference in the distribution of genotypes between the obesity group and control group. The result was same after several influence factors controlled. （2）The levels of HDL were significantly different among genotype groups. Subjects for the B2 B2 genotype had the highest HDL levels. The relationship was steady after adjusting several influence factors. （3）Subjects for the B2B2 genotype had higher HDL level after intervention, which was significantly different to other genotype groups. After adjusting baseline HDL level and gender, genotype didn＇t effect the change in HDL. Conclusion CETP-TaqIB gene polymorphism influenced serum HDL level. But this gene polymorphism at CETP locus wasn＇t especial in adult obesity. Baseline HDL level influenced the change in HDL response to dietary intervention in three genotype groups.