早产儿母亲血浆铜和胎膜胶原含量初步分析

Analysis of the concentration of plasma copper and collagen in fetal membrane of the preterm's mothers

目的对早产儿母亲血浆铜和胎膜胶原含量进行初步研究,探索二者的联系,为研究早产的发生机制提供重要线索。方法分别收集早产和足月新生儿及其母亲的一般情况、ICP-OES检测两组母血和脐血血浆铜含量。首次对胎膜胶原采用天狼红染色,图像分析比较两组胎膜厚度和Ⅰ型、Ⅲ型胶原的含量。结果胎膜早破在早产儿母亲和足月儿母亲的发生率分别为40·00%和9·09%。早产儿母亲孕前体重、早产儿身长、出生体重明显低于足月儿母亲和足月儿(P<0·05)。早产儿脐血血浆铜显著低于足月儿(P<0·05)。足月儿母亲非破裂处胎膜厚度显著大于早产儿母亲(P<0·05),而且也显著大于其自身破裂处胎膜厚度(P<0·05)。早产儿母亲非破裂处胎膜的Ⅰ型、Ⅲ型胶原含量显著低于足月儿母亲(P<0·05)。结论早产儿母亲血浆铜和胎膜胶原含量都低于足月儿母亲。

Objective The purpose of the study is to determine primarily the concentration of plasma copper and collagen in fetal membrane of the preterm＇ s mothers, and explore the relation of copper status and fetal membrane collagen of preterm＇s mothers. Methods The general situations were collected both in neonatos and their mothers, The plasma copper concentrations of mother venous blood and cord blood were analyzed by ICP-OES. Sirius Red dyeing was first used in fetal membrane to detect the thickness and collagen in preterm＇s and full-term＇s mothers. Results The prevalence of premature rupture of membrane （PROM） was 40.00%in preterm＇s mothers, and 9.09% in the full-term＇s mothers. The mothers＇ preconceptual weight, infant body length and body weight at birth were lower in preterm group than those of the full-term group. The cord blood plasma copper was significantly lower in preterms than in full-terms. Both the thickness and the concentration of collagen Ⅰ and Ⅲ of non-split fetal membrane of preterm＇s mothers were significantly less than those of the full-term＇ s mothers. Conclusion Both the concentration of plasma copper and fetal membrane collagen of preterm＇s mothers were lower than that of the full-term＇ s. It can confer that the deficient copper status could lead to immature collagen in fetal membrane which induce PROM and have preterm birth finally. More work need to be done in this field.