微藻叶绿素荧光值与传统生长指标的关联性研究

The study of using TD-700 Fluorometer to determine the biomass of microalgal

利用TD-700型叶绿素荧光仪测定了6种不同海洋和淡水微藻,即锥状斯氏藻(Scrippsiellatrochoidea)、球形棕囊藻(Phaeocystisglobosa)、三角褐指藻(Phaeodactylumtricornutum)、眼点拟微绿球藻(Nannochloropsisoculata)、四列藻(Tetraselmistetrethele)和雨生红球藻(Haematococcuspluvialis)的叶绿素荧光值,同时采用经典方法同步测定其细胞密度和叶绿素a含量。统计分析结果显示,微藻细胞密度、叶绿素a含量、叶绿素荧光值三者之间具有显著或极显著的正相关关系。因此,利用叶绿素荧光仪测定微藻生物量的方法是切实可行的,具有快捷方便、灵敏度高的优点。

Six microalgaes, including Scrippsiella trochoidea, Phaeocystis globosa, Phaeodactylum tricornutum, Nannochloropsis oculata, Tetraselmis tetrethele and Haematococcus pluvialis were studied.Their fluorescent value of different kinds and concentrations of microalgaes were determined with using TD-700 Fluorometer. Compared with cell counting and chlorophyll-a content measure, it shows a very good linear interrelation between the cell density, chlorophyll-a content and fluorescent value. So the method using TD-700 Fluorometer to determine the microalgal biomass is a quick and reliable method which can reduce artificial error, increase high consistency and operational simplicity.