摘要
目的探讨卵巢切除和衰老对大鼠基底前脑的Meynert基底核和斜角带水平肢胆碱能神经元的影响。方法利用乙酰胆碱转移酶免疫组织化学方法观察正常大鼠、卵巢切除大鼠和老年大鼠的胆碱能神经元数量、胞体大小和突起的变化。结果卵巢切除组动物Meynert基底核和斜角带水平肢的ChAT免疫阳性神经元的细胞数(37±5,126±24),胞体平均面积(107.33±21.45μm2,101.50±25.88μm2),突起数目(1.7±0.5,1.5±1.0)均低于正常成年组ChAT免疫阳性神经元的细胞数(56±8,164±20),胞体平均面积(167.17±40.12μm2,193.33±44.59μm2)和突起数目(3.0±0.6,3.0±0.9),差异有显著性(P<0.05)。老年组的ChAT免疫反应神经元细胞数(39±6,126±15),胞体平均面积(108.00±22.10μm2,95.17±29.64μm2),突起数目(2.0±0.9,1.8±0.8)与正常组比较同样具有明显的差异性(P<0.05)。结论卵巢切除和衰老引起大鼠基底前脑的胆碱能神经元胞体变小、数量减少、突起数目明显减少,提示雌激素缺乏能导致基底前脑的胆碱能神经元溃变、丢失,可能与AD病人出现的学习记忆和认知功能障碍有关。
Objective To explore the effects of ovariectomy and aging on cholinergic neurons from rat basal nucleus of Meynert and horizontal limb of diagonal band in the basal forebrain. Methods The adult, aged and ovariectomized experimental rats were enrolled in the study. ChAT immunoreactivity was applied to the histologic investigation. Results In the basal nucleus of Meynert and horizontal limb of diagonal band, a significant reduction occurred in the number (37 ± 5 vs. 126 ±24), size (107.33±21.45 μm^2 vs. 101.50±25.88μm^2) and the number of cell processes (1.7±0.5 vs. 1.5±1.0) of ChAT- immunoreative neurons in the ovariectomized group as compared with the normal adult female rats (P〈 0.05). Significant differences were also identified in the number of cells (56 ± 8 vs. 164 ± 20 ; 39 ± 6 vs. 126± 15), size ( 167.17 ±40.12μm^2 vs. 193.33 ± 44.59μm^2 ; 108.00± 22.10μm^2 vs. 95. 17 ± 29.64μm^2 ) and the number of cell processes (3.0 ± 0.6 vs. 3.0±0.9; 2.0±0.9vs. 1.8±0.8) between the normal adult and aged rats (P〈0.05). Conclusions Significant morphological changes of CHAT- immunoreative neurons in the basal forebrain and their cell processes occur in ovariectomized and aged rats,and it is suggested that deprivation of estrogen resulted in cholinergic neurons degeneration in basal forebrain, which might be involved in cognitive deficit and learning and memory dysfunction.
出处
《实用预防医学》
CAS
2006年第4期841-843,共3页
Practical Preventive Medicine