抑瘤宁对食管癌细胞系Eca-109作用的研究

The Research on the Effect of Yiliuning on Esophageal Cancer Cell Eca-109

目的研究抑瘤宁对食管癌细胞系Eca-109的生长抑制作用及其机制。方法采用MTT法检测抑瘤宁及其组方中各单药对体外培养Eca-109细胞的生长抑制作用;采用流式细胞仪检测细胞周期分布及凋亡率。结果抑瘤宁较组方中各单药更能明显的抑制Eca-109细胞的生长,流式细胞仪检测结果显示,各实验组S期细胞百分率明显升高,G0/G1期细胞百分率明显下降(其中各单药组、抑瘤宁组与对照组比较,P<0·01;各单药组与抑瘤宁组比较,P<0·01),细胞被阻滞于S期,细胞凋亡率升高。结论抑瘤宁对Eca-109细胞的增殖具有明显抑制作用,其机制可能与细胞周期阻滞和凋亡有关。

Objective To study the proliferation inhibition effect and mechanism of Yiliuning on esophageal cancer cell Eca-109.Methods By means of the MTT method to test the proliferation inhibition effect of Yiliuning and its single medicine on esophageal cancer cell Eea-109; and by means of the flow eytometry to test the distribution of cells cycles and apoptosis rate. Results It obviously inhibited the Eea- 109 cells＇ growing to use Yiliuning other than single medicine, the results of flow cytometry analysis showed that the S-phase cells＇ percentage of every experimental group was obviously increased and the cells＇ percentage from G0/G1-phase was obviously decreased （in the percentage each single medicine group, the Yiliuning group compared with the control group, P 〈 0.01 ;and each single medicine group compared with the Yiliuning group, P 〈 0.01 ）, cells were retarded in S-period, the percentage of cells apoptosis were increased. Conclusions Using the Yiliuning had the inhibition of Eca-109 cells＇ proliferation, and its mechanism was relevant to the retardant of cells＇ cycle and apoptosis.