基因芯片技术分析老年冠心病患者的易感基因

Relationship of angiotensin-converting enzyme, angiotensinogen and endothelial nitric oxide synthase gene polymorphisms with coronary disease in aged people

目的研究血管紧张素转换酶(ACE)、血管紧张素原(AGT)及内皮型一氧化氮合酶(eNOS)基因多态性与老年人冠心病(CHD)的关系。方法选择老年CHD患者100例及对照者91例,应用基因芯片技术检测ACE、AGT和eNOS基因多态性,并比较其基因型及等位基因频率。结果CHD组ACE DD基因型频率(28．0％)与对照组(15．4％)比较,差异有统计学意义(P< 0．05),ACE基因多态性与老年CHD相关。AGT TT基因型频率(75．0％)与对照组(51．7%)比较,差异有统计学意义(P<0．01),AGT基因多态性与老年CHD相关。eNOS TT基因型频率(5．0％)与对照组(0．0％)比较,差异无统计学意义(P>0．05)。同时携带ACE DD和AGT TT基因型或AGT TT和eNOS TT基因型者与老年CHD呈显著正相关(OR=2．9,P<0．05,OR=1．1,P< 0．05)。结论ACE和AGT基因多态性可能是中国老年人CHD的危险因素。

Objective To evaluate the predisposition effects of angiotensin-converting enzyme （ACE）, angiotensinogen （AGT） and endothelial nitric oxide synthase （eNOS） gene polymorphisms on coronary disease（CHD） as well as their possible synergistic effect in the development of CHD in Chinese aged people, Methods The study included 191 subjects （100 CHD and 91 controls）, Gene chip technology was performed, The allele frequency and genotype distribution were compared between groups, Results A significantly higher frequency of the DD genotype of ACE gene was observed in CHD group （28.0% vs 15.4%, P〈 0. 05 ）than in control group. The ACE gene polymorphism was associated with CHD, A significantly higher frequency of the TT genotype of AGT gene was observed in CHD group（75.0% vs 51.7%, P〈0.01）than in control group. The AGT gene polymorphism was associated with CHD. No significant difference was observed in eNOS genotype distribution between the two groups （5.0%.A vs 0. 0%, P〉0. 05）. Subjects carrying ACE DD and AGT TT genotypes or those carrying AGT TT and eNOS TT genotypes showed a stronger association withCHD（OR=2.9, P〈0.05, OR=I. 1, P〈0.05, respectively）. Conclusions The ACE and AGT gene polymorphisms may be risk factors of CHD. Gene chip technology provides an effective and sensitive method to study the relationship of gene polymorphisms and CHD.