蛇毒降纤酶对兔眼外伤性PVR层粘连蛋白、Ⅳ型胶原的影响

The effect of defibrase on laminin and Ⅳ collagen in traumatic rabbit eyes with proliferative vitreoretinopathy

目的:探讨蛇毒降纤酶对外伤性增生性玻璃体视网膜病变(PVR)玻璃体腔中的层粘连蛋白(lam in in,LN)、Ⅳ型胶原(Ⅳco llagen,ⅣC)的影响。方法:对20只兔子采用兔眼后节穿通伤加注血法或/和加蛇毒降纤酶制备实验模型,随机分成正常对照组(20眼),全血组(10眼),蛇毒降纤酶组(10眼)。利用免疫组化SP法观察实验各组,检测PVR玻璃体腔增殖膜中LN、ⅣC的含量。结果:蛇毒降纤酶组增殖膜中LN、ⅣC阳性面积比值分别为0.5931±0.15786,0.5354±0.19216;全血组LN、ⅣC阳性面积比值分别为3.8271±1.3537,1.4288±0.66307。蛇毒降纤酶组增殖膜中LN、ⅣC的阳性表达均低于全血组(P<0.01)。结论:蛇毒降纤酶能够降低PVR增殖膜中LN、ⅣC的阳性表达,抑制PVR的发生、发展。

Objective： To study the effect of defibrase on laminin and Ⅳ collagen in experimental traumatic proliferative vitreoretinopathy. Methods： Twenty experimental models were induced by posterior penetrating eye injury and vitreous infusing blood or/and defibrase in the rabbit. These rabbits were divided into three groups randomly： normal control group （twenty eyes）, full blood group （ten eyes）, and defibrase group （ten eyes）. The positive expression of LN, Ⅳ C in the proliferative membranes were examined by the immunohistochemical SP. Results： Compared with the full blood group, the positive expression of LN, Ⅳ C in the proliferative membranes were decreased in the defibrase group （P〈0.01）. Conclusion： Defibrase can decrease the positive expression of LN, Ⅳ C in the proliferative membranes, inhibit the development of PVR.