植物蛋白质酶解产物超滤分析及对成纤维细胞生长的影响

Ultrafiltrating analysis of plant protein hydrolysates and the effect of hydrolysates on the growth of the mouse embryo fibroblasts

用木瓜蛋白酶、胰蛋白酶和A s1.398枯草蛋白酶分别对豆粕、棉粕、菜粕和花生粕的蛋白质进行酶解,并对其酶解产物进行超滤分析及用产物对小鼠胚胎成纤维细胞进行培养试验,探讨不同蛋白酶解产物的蛋白质组成及对细胞生长的影响。结果表明:酶解产物中蛋白质分子质量分布与酶的种类和植物蛋白质种类有关,其中A s1.398枯草蛋白酶对豆粕酶解能力最强,酶解产物中蛋白质分子质量小于3 ku的比例占31.67%;各酶解产物对小鼠胚胎成纤维细胞生长都有促进作用,以A s1.398枯草蛋白酶酶解的棉粕产物对小鼠胚胎成纤维细胞生长的促进作用最好,其细胞生长速度比豆粕、菜粕和花生粕酶解产物组显著提高。

Papain, trypsin and Asl. 398 subtilisin were used to degrade .soybean meal, cottonseed meal, rapeseed meal and peanut meal separately, then used enzymatic degradation products to ultrafiltrate and to study the effect on the cell culture of the mouse embryo fibroblasts. The results indicated that the distribution of the molecular weights of the enzymatic degradation products was related to the kinds of the enzymes and the substrates, and Asl. 398 subtilisin was the most degraded enzyme for the soybean meal, the percentage of the molecular weight which was less than 3 ku in the enzymatic degradation products was 31.67%0; all enzymatic degradation products could promote the growth of the mouse embryo fibroblasts, and the most effective enzymatic degradation products was the cottonseed meal degraded by Asl. 398 subtilisin, the speed of cell growth of that was higher than that of enzymatic degradation products of the peanut meal, rapeseed meal and soybean meal.