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甘蔗种质资源AFLP研究的引物筛选

Primers Screening for Sugarcane(Saccharum officinarum L.) Germplasm AFLP Markers
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摘要 以两个甘蔗品种ROC25和ROC23的基因组DNA为模板,利用AFLP技术从110对引物中筛选出5对带型分布均匀、多态性丰富且分辨能力强的选择性引物。5对引物分别是E2/M4,E4/M5,E5/M6,E11/M7和E2/M6,它们将为以后AFLP技术应用于甘蔗遗传研究提供参考。 The genomic DNAs of the ROC25 and ROC23 were analyzed with the technology of Amplified Fragment Length Polymorphism （AFLP）. Five pairs of AFLP primers were selected from the 110 pairs primer which had rich polymorphism for the genomic DNA of sugarcane. They are the E2/M4, E4/M5, E5/M6, E11/M7 and E2/M6, which provide a reference for sugarcane genetic research with AFLP.
作者 梁朝旭 李鸣 梁俊 方位宽 李杨瑞 何为中 谭裕模
机构地区 广西甘蔗研究所 广西作物遗传改良生物技术重点开放实验室
出处 《中国糖料》 2006年第3期1-4,共4页 Sugar Crops of China
基金 广西科学基金(0342007-2) 广西农科院基金(2002022)资助
关键词 甘蔗 AFLP 引物筛选 Sugarcane Amplified Fragment Length Polymorphism （AFLP） Primer screening
分类号 S566.1 [农业科学—作物学]
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参考文献19

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