室内装修对人体及小鼠外周淋巴细胞DNA损伤的影响

Effect of Volatile Organic Compounds in Indoor Renovation on the DNA Damage in Peripheral Blood Lymphocytes in Human and Mice

目的研究新装修客房中挥发性有机毒物对人体及小鼠外周淋巴细胞DNA损伤的影响。方法通过动物实验和人群流行病学调查相结合的方法,应用单细胞凝胶电泳法,分别检测在新装修场所和未装修场所工作半年以上女服务员外周血淋巴细胞DNA的损伤和实验动物外周血淋巴细胞DNA的损伤。结果在新装修场所工作半年以上女服务员的外周血淋巴细胞DNA断裂比对照组高,差别具显著性意义(P<0.01)。动物实验组外周血淋巴细胞DNA的损伤高于对照组,两组比较具有显著性差异(P<0.01)。结论新装修住房中挥发性有机毒物对人体外周血淋巴细胞存在DNA损伤作用。高浓度有机挥发物可引起小鼠外周血淋巴细胞DNA损伤。

Objective To explore the DNA damage in peripheral blood lymphocytes caused by exposure to the volatile organic compounds emitted from indoor renovation. Methods The study was included both of animal experiments and epidemiological survey. The DNA damage was studied with single cell gel eleetrophoresis assay in peripheral blood lymphocytes obtained from waitress working over half year in newly renovated workplaees and unrenovated workplaees. The animal experiment was also carried out for investigation of DNA damage in peripheral blood lymphocytes of exposed mice.Results The rate of DNA breaks in peripheral blood lymphocytes of waitress working in newly renovated workplaees was significantly higher than those of the negative control group （P〈0.01）. Moreover, the DNA damage in peripheral blood lymphocytes of exposed mice was significantly higher than those of the negative control group （P〈0.01）. Conclusion The DNA damage was existed in the peripheral blood lymphocytes of human exposed to the volatile organic compounds emitted from newly renovated areas. The high concentration of volatile organic compounds could cause the DNA damage in peripheral blood lymphocytes of exposed mice.