不同实验方法在人胎脑海马神经干细胞检测中的应用

Application of Different Experimental Methods for Determining Neural Stem Cells in Hippocampus from Human Fetal Brain

目的探讨精确可靠的检测人胎脑神经干细胞(NSCs)的方法。方法收集胎龄32周的水囊引产胎儿10例,灌注固定后分别予石蜡包埋和冷冻切片,采用HE检测Nestin蛋白表达免疫组织化学染色和光镜技术对人胎脑海马部位两种切片组织结构的改变和NSCs进行检测。结果石蜡切片海马组织Nestin蛋白表达阳性NSCs数较少,每个NSCs中Nestin蛋白表达的量也较少:而冷冻切片Nestin蛋白表达阳性NSCs数较多,且每个NSCs中Nestin蛋白表达的量也较多,石蜡切片中组织及细胞结构完整性优于冷冻切片。结论水囊引产、灌注固定及冷冻切片三结合研究人胎脑组织NSCs,是一种可靠、精确而敏感的实验方法。

Objective To explore a precise and dependable method for determining neural stem cells（NSCs）in hippocarnpus from human fetal brain. Methods Ten cases of embryo at gestational age 32 weeks and induction of labor with water bag were collected, affused and sliced including frost slice and paraffin slice for determining alteration of tissue and NSCs in hippocarnpus from human fetal brain with HE stain and immunohistochemical staining under light microscope. Results Nestin proteins located in NSCs and the number of NSCs were less in paraffin slice of tissue than those in frost slice. Integrality of structure of tissues and cells in paraffin slice excelled in frost slice. Conclusions There is a precise and dependable method including induction of labor with water bag and allusion and frost slice, which is necessary for wide useness in study of NSCs in human fetal brain.