大孔吸附树脂一次性分离杜仲叶中杜仲总苷和杜仲黄酮的研究

Isolation of total glycosides and flavonoids from Eucommia ulmoides leaf by macroporous resin

本文就9种大孔树脂吸附分离杜仲总苷和杜仲黄酮的工艺进行了研究,结果表明:一次性分离杜仲总苷(富含绿原酸、桃叶珊瑚苷等活性物质)和杜仲黄酮的适宜的大孔树脂为XDA-1和X-5。适宜的吸附解吸条件分别为:上柱液pH 6,吸附时间应大于10 h;XDA-1树脂用40%乙醇洗脱杜仲总苷,用80%的乙醇洗脱黄酮;X-5树脂用30%的乙醇洗脱杜仲总苷,用70%的乙醇洗脱黄酮。两种树脂的分离效果分别为:XDA-1分离杜仲总苷(其中绿原酸含量为14.23%,桃叶珊瑚苷含量为7.69%)粗品得率为8.01%,杜仲总黄酮粗品得率为4.76%,含量为15.82%;X-5分离杜仲总苷(其中绿原酸含量为15.39%,桃叶珊瑚苷含量为9.07%)粗品得率为7.35%,杜仲总黄酮粗品得率为5.11%,含量为16.15%。

Technology for the isolation of total glycosides and flavonides from the leaves of Eucommia ulmoides by 9 kinds of macroporous resins was studied. Results show that the optimum resins are XDA-1 and X-5. The technical conditions for the separation are as follows, pH value of the sample solution loaded. 6, static absorption time. above 10 h, suitable desorption solvent to XDA-1. 40% ethanol for chlorogenic acid and aucubin, 80% ethanol for flavonoids; to X-5： 30% ethanol for chlorogenic acid and aucubin, and 70% ethanol for flavonids. The yield of total glycosides （crude product） extracted by using XDA-1 is 14. 23%, in which the content of chlorogenic acid is 14. 23%, of aucubin, 7. 69%. The yield of flavonoids （crude product） extracted by using XDA-1 is 4.76% with the content of 15.82%. The yield of total glycosides （crude product） extracted by using X-5 is 7. 35%, in which the content of chlorogenic acid is 15.39%, of aucubin, 9.07%. The yield of flavonoids （crude product） extracted by using X-5 is 5.11% with the content of 16. 15%.