PCR方法检测鸡减蛋综合征病毒

Detection of egg drop syndrome 76 virus by PCR assay

根据已报道的鸡减蛋综合征（Egg drop syndrome 1976EDS-76）病毒DNA序列，设计合成了1对能扩增300bpDNA片段的引物，建立了EDS-76病毒的聚合酶链式反应（PCR）诊断方法。该方法对EDS-76病毒标准株AV-127扩增结果为阳性；对鸡传染性支气管炎病毒（IBV）、鸡传染性法氏囊病病毒（IBDV）和鸡新城疫病毒（NDV）的扩增结果均为阴性。结果表明该方法特异且敏感，检测的最低病毒DNA含量达到2．5×10^-2pg，PCR检测表明，从试验感染鸡的心脏中不能检测出病毒，肝脏和肾脏只有在感染后7～15d检出，在试验感染后4～21d的子宫、血液和4～17d的脾中均能检测到病毒。

Polymerize chain reaction （PCR） assay was developed for detection of EDS - 76 virus. A pair of primers was synthesized based on the DNA sequence of EDS- 76 virus. An expectation of 300bp DNA fragment of EDS- 76 virus was amplified by PCR using the primers. The PCR assay is an effective method used to diagnose EDS- 76 virus. It could detect as little as 2.5 - 10^-2pg of purified DNA of EDS- 76 virus. The crossing - reaction test and sensitive - reaction test indicated that PCR is sensitive and specific. The PCR test showed that the EDS - 76 does not exist in the heart, but exists during 7-15 day post infection （DPI） in liver and kidney, 4--21 DPI in uterus and blood and 4--17 DPI in spleen.