肿瘤坏死因子-α基因多态性与多发性硬化的相关性

Association between tumor necrosis factor-α gene polymorphisms and Multiple Sclerosis (MS) in Han nationality of Guangdong.

目的对广东地区汉族多发性硬化症(MS)患者作肿瘤坏死因子-α(tumor necrosis factor,TNF-α)基因分型,探讨TNF-α基因与广东人群中MS遗传易感性的可能关系。方法采用用聚合酶链式反应-限制性片段长度多态性技术对68例无亲缘关系的MS的病人和106例无血缘关系的广东籍健康汉族人作TNF-α分型。结果TNF-α各等位基因基因型频率在MS组和正常人组间无显著差(P均>0.05)。结论从目前调查的例数分析,广东人群中MS与TNF-α基因不关联。

Objective To investigate the distribution of TNF - α genes in a Guangdong population, examined the association of these genes with multiple sclerosis. Methods The normal subjects consisted of 106 unrelated healthy individuals of Han nationality from the Guangdong region. 68 patients with multiple sclerosis, all from the same geographic region,TNF - α were genotyped by the PCR - RFLP method,TNF - α genotypes were assigned according to the electrophoretic patterns of the nucleotide fragments produced by NcoI digestion of the specific PCR products. Results There was no significant differences in allelic frequencies of TNF -α gene between the patients and normal controls. Conclusion The polymorphisms of TNF - α genes is not associated with MS in Guangdong region.