采用放射性骨显像评价rhBMP-2与珊瑚人工骨复合植入狗牙槽骨后的成骨效能

Evaluation of repair of alveolar bone defects with coral artificial bone composite of human recombinant bone morphogenetic protein-2 using dynamic ^(99)Tcm-MDP bone imaging.

目的探讨应用99Tcm-MDP放射性骨显像评价重组人骨形成蛋白-2(rhBMP-2)与珊瑚人工骨在体外复合后植入狗缺损牙槽骨中后的成骨效应。方法拔除12只成年狗两侧上颌第二及第三切牙,并去除牙槽窝之间的牙槽间隔,一侧随即植入复合骨,对侧植入珊瑚骨作为对照。并于分别于植入后4、8、12周后取材,采用组织学观察、图像分析和99Tcm-MDP核素骨显像等方法比较两种类型的骨移植材料在牙槽窝中的骨修复能力。结果复合骨植入牙槽骨后,材料被逐渐降解吸收,新骨不断生成,12周后,植入材料完全被成熟的骨组织取代;图像分析结果显示不同时间复合骨组新骨形成的比值显著高于单纯珊瑚骨组(P<0.05);4周和8周复合骨组核素浓聚程度高于珊瑚骨组,12周两组核素浓聚程度差异不明显。结论99Tcm-MDP放射性骨显像能早期判断移植骨的成活情况及成骨效果。

Objective To study the value of ^99Tcm - MDP bone scanning in monitoring the survival and bone repairing ability of coral artificial bone composite of rhBMP -2 （rhBMP -2/CAB） implanted into extraction sockets. Methods 12 adult dogs served as the experimental animals. Immediately after extraction of the upper second and third incisors, RhBMP -2/CAB and coral artificial bone （CAB） were respectively implanted into each site of extraction sockets. The bone repairing ability of the two grafts was analyzed with histology, image analysis system and radionuclide bone imagining at 4th, 8th and 12th week respectively. Results RhBMP - 2/CAB were absorbed gradually after they were implanted into alveolar bone defect and were replaced completely by bone at 12 weeks. The ratio of new bone formation of rhBMP -2/CAB was significantly higher than that of CAB at different time （ P 〈 0.05 ）. The uptake of ^99Tcm - MDP in rhBMP - 2/CAB was higher than that of CAB at 4 and 8 weeks but there were no differences between the two groups at 12 weeks. Conclusion The repairing ability and effect of rhBMP - 2/CAB in extraction sockets are obviously better than those of CAB and radionuclide bone imaging the chief method for early assessment of the survival of bone grafts.