高氧液治疗窒息后新生儿缺氧缺血性脑病前后血清相关因子变化的探讨

Discussion on the changes of relevant serum-parameters in neonatal HIE treated by medical pressured oxygen injector after asphyxia.

目的探讨高氧液对新生儿窒息后所致新生儿缺氧缺血性脑病（HIE）治疗的可行性及疗效。方法对51例HIE患儿用高氧液治疗前10～20min及治疗后30min、48h、72h，分别采用直接比色法检测患儿血清相关因子超氧化物歧化酶（SOD）、丙二醛（MDA）、谷胱甘肽过氧化物酶（GSH—PX）、一氧化氮（NO）水平的变化。结果用高氧液前，轻度HIE和中重度HIE两组SOD、GSH—PX水平明显降低，MDA、NO明显升高，用高氧液后与各自治疗前相比差异有显著性。结论高氧液治疗不能导致患儿自由基生成增多及脂质过氧化损伤；而是可达到清除氧自由基的作用，保护细胞免受损伤。由于样本少，高氧液的作用机制和在儿科的临床应用有待于进一步研究和探讨。

Objective To investgate feasibility and effectiveness of medical pressured oxygen injection （ MPOI, Vanform Biomedical Engineering Technology Co, LTD） in the treatment of neonate with hypoxic - ischemic encephlopathy（HIE） after asphyxia. Hypoxic - ischemic related serum parameters superoxide dismutase （ SOD）, malondiadehyde （ MDA）, glutathione peroxidase （ GSH - PX） and NO （ Nitric Oxide） were measured before and after MPOI infusion. Methods Serum SOD, MDA, GSH -PX and NO were measured in 51 neonatal with HIE 10 -20 minutes before and 0.5, 48 and 72 hours after MPOI infusion by use of direct chromatometry. Results Serum SOD and GSH - PX were significantly decreased and serum MDA and NO were significantly increased before MPOI infusion, after MPOI infusion all the four hypoxic - ischemic related serum parameters improved significantly. Conclusion The study shown that there was significant changes in four hypoxia - ischemic related serum parameters after MPOI infusion compared with baseline values; It seems that MPOI may not result in an increase in free - radical production and peroxidatic injury of lipid but can clear free - radicals and protect brain cells form hypoxic - ischemic injury. The study limitation is that sample size is too small. Further studies are needed to elucidate its mechanism of action and clinical usefullness.